The Biotechnology Department awarded a Master's degree to graduate student "Hanin Salah Lazem."
The Biotechnology Department awarded a Master's degree to graduate student "Hanin Salah Lazem" for her thesis titled...
“Gene Expression alterations, Immunological,
and Biochemical studies of Colorectal Cancer and Hepatocellular carcinoma in Iraqi populations “
The defense was held in the late Prof. Dr. Abdulmutalib Ibrahim Al-Sheikh Hall in the department building, and the examination committee consisted of...
- Prof. Dr. Majid Sakhy Jabir
- Department of Applied Sciences / Biotechnology Branch – Chair
- Prof. Dr. Dunia Farid Salloum
- niversity of Baghdad / College of Science / Department of Life Sciences – Member
- Assist. Prof. Dr. Hiba Munir Abdul-Hussein
- Department of Applied Sciences / Biotechnology Branch – Member
- Assist. Prof. Dr. Zahraa Abdulmuneem Abdulhadi
- Department of Applied Sciences / Biotechnology Branch – Member and Supervisor
- Prof. Dr. Ghassan Mohammed Suleiman
- Department of Applied Sciences / Biotechnology Branch – Member and Supervisor
The present work intended to explore the pathogenesis role of these parameters on CRC patients, namely interleukins 18 (IL-18) and 22 (IL-22), Kelch-like ECH associated protein-1(keap-1), Aldolase A(ALDOA), and forkhead box O4 (FOXO4) in this disease. The RT-qPCR method was used to measure FOXO4 gene expression, while an ELISA kit was used to quantify IL-18, IL-22, Keap-1, and Aldolase levels. Research was performed on 60 patients with CRC and 30 healthy controls. IL-18, IL-22, and Keap-1 were not statistically significant in CRC cases with the variant size of tumor stages, histological grades, and different situations of distant metastasizing tumor cells (P>0.05). While ALDOA shows highly significant variations between patients and apparently healthy subjects (P<0.05). The expression of FOXO4 is down-regulated and was statistically different (P<0.05) in patients with CRC. Analytical indicators showcasing the inflammatory state include metrics like (SII), (PLR), and (NLR) which have been suggested as instruments for assessing the progression of colorectal cancer (CRC). Evaluating the efficacy of these indicators, along with a novel indicator that merges SII and hemoglobin levels, referred to as NP/LHB, as tools for CRC diagnosis. PLR and NLR show non-significant differences (P>0.05), and SII, NP/LHb show significant variations between CRC patients and the control group (P<0.05). Hb, WBC, Lymphocyte, and Neutrophil show a non-significant variation between patients and the healthy subjects group (P>0.05), and PLT count shows a significant variation between the patient and control group(P<0.05). The three liver enzymes are aspartate V transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP). ALT and AST show a significant increment in patients than the healthy subjects group (P<0.05), ALP shows a non-significant between CRC patients and healthy subjects (P>0.05), RFT which includes Serum creatinine (S.cr) and blood urea nitrogen (BUN). Creatinine shows a significant decrease in patients than healthy subjects (P<0.05).
