The Department of Applied Chemistry awarded a master's degree to the graduate student "Fatima Qasim Hussein"
The Department of Applied Chemistry awarded a master's degree to the graduate student "Fatima Qasim Hussein" for her thesis titled...
“Evaluating Anti-Acetylated Peptide Antibodies and Some Biochemical Parameters in Serum of Patient with Rheumatoid Arthritis
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he defense was held in the late Professor Dr. Abdul Muttalib Ibrahim Al-Sheikh Hall in the department building, and the examination committee consisted of...
- Professor Dr. Bushra Fares Hassan
- University of Baghdad / College of Science for Women Chair
- Assistant Professor Dr. Mohammed Shamil Ali
- Department of Applied Sciences / Applied Chemistry Branch Member
- Assistant Professor Dr. Wafaa Raji Mohammed
- Department of Applied Sciences / Applied Chemistry Branch Member
- Professor Dr. Abdul Nasser Mohammed Abdullah
- Department of Applied Sciences / Applied Chemistry Branch Member and Supervisor
- Professor Dr. Mohammed Hadi Munshid Al-Asami
- University of Baghdad / College of Medicine Member and Supervisor
One hundred ten RA patients (13. 63 % male, 86.36% female) with a mean age of 23.8±6.8 years and while forty were apparently healthy control individuals with no signs or history of any autoimmune diseases or malignancies were involved in this study that was conducted in the period between Sep.2022 to Jan. 2023. The Patients divided into three subgroup: (1) biological treatment subgroup (consisted of thirty -eight patients on the bDMARDs treatment), (2) conventional cDMARD subgroup [thirty - eight patients received Methotrexate (MTX)] and (3) combined DMARD subgroup (thirty –four patients had received biology and chemotherapy). The human anti citrullinated peptide antibodies (ACPAs), human anti acetylated peptide antibodies (AAPAs), human acetylated Lysine antibodies (AcLys), human anti acetylated ornithine antibodies (AcOrns) were determined by Enzyme Linked Immunosorbent Assay (ELISA) and Zinc, Copper, LDH and alkaline phosphatase levels were used to measure by spectrophotometer in the serum for RA patients and apparently healthy controls. The results reveal that a high significant increase in prevalence of ACPA in RA more than apparently healthy. There are three acelyted peptide antibodies (AAPA, AcLys and AcOrn) are highly prevalent in RA and occur also independently of RF and ACPA. Moreover, high significant decrease difference in the serum level of AcLys (P<0.001) in chemotherapy group in comparison to other subgroup as well as a significant decrease (p=0.0149 ) in the serum levels of AcOrn between Biology vs. Chemotherapy RA patients subgroups. Significant decrease in serum Zn levels in healthy control compared to the sub group of RA patients. A high significant increase in serum Cu levels in sub group of RA patients compared to the healthy control. Moreover, High significant level of LDH in RA compared to healthy control and high significant decrease LDH in biology subgroup compared to other treatment subgroup of RA. the positive correlation between ACPA with AAPA, AcLys and with dieses activity score 28 (DAS28). In conclusion, the evaluating of autoantiody (ACPA,AAPA,AcLys, AcOrn) in RA and multiple AAPA reactivity increased the specificity for RA, suggesting high diagnostic value of AAPA testing. Furthermore, Cu and LDH as a marker for inflammation in RA.
